Normalize nanostring gene expression using common pools between two CodeSets.
Usage
normalize_pools(x, x_pools, ref_pools, p = 3, weigh = TRUE)
Arguments
- x
target data
- x_pools
target pool samples
- ref_pools
reference pool samples
- p
number of pool sample sets. Defaults to 3.
- weigh
logical; if TRUE, the average expression in x_pools is
reweighed by the distribution of the p pool sample sets in ref_pools.
Value
normalized gene expression
Details
The target and reference expression samples, as well the target and reference
pool samples all need to be specified. We recommend reweighing the target
pool samples when calculating the average expression by the distribution of
reference pools.